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pbabe krasg12d plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pbabe krasg12d plasmid
    Pbabe Krasg12d Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+krasg12d+plasmid/pBabe-Kras+G12D+(Plasmid+%2358902)/bio_rxiv__64898__2026__03__24__711280-204-3-11
    Average 93 stars, based on 41 article reviews
    pbabe krasg12d plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Cell-of-Origin, not Oncogenic Effect, Determines Desmoplastic Immune Exclusion in KRAS-Driven Liver Cancer
    Article Snippet: .. [ ] The pBabe-KrasG12D plasmid was reconstructed from pBabe-Kras G12D -puro (Addgene #58902) by removing the puromycin resistance gene through digestion with HindIII-HF and BspDI, blunt-ended by Klenow and re-ligation to restore plasmid circularity. ..

    Article Title: A systematic genome-wide mapping of oncogenic mutation selection during CRISPR-Cas9 genome editing
    Article Snippet: .. Constructs and stable cell lines MOLM13 cells were obtained from DSMZ (Cat. ACC-554) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA) supplemented with 10% v/v heat-inactivated fetal bovine serum (Sigma-Aldrich, Saint Louis, MI), 2 mM L-Glutamine (LifeTechnologies) and 100 U/mL penicillin/streptomycin (LifeTechnologies). p53 R248Q was PCR ampli ed from a bacterial expression plasmid (kind gift of Dr. Shannon Lauberth, UCSD) and KRASG12D the pBabe-KRASG12D plasmid (Addgene plasmid 58902, from Dr. Channing Der) using the Kappa Hi- delity DNA polymerase (Kappa Biosystems). ..

    Article Title: Therapeutically reprogrammed nutrient signalling enhances nanoparticulate albumin bound drug uptake and efficacy in KRAS-mutant cancer.
    Article Snippet: KP or iKras cells stably expressing ERK-KTR, referred to as KP ERK-KTR or iKras ERK-KTR, respectively, were selected with growth media containing 10 μg ml−1 puromycin (Invitrogen) for 7 d and subsequently sorted for cells with high expression of fluorescent proteins via fluorescence activated cell sorting (FACS, MGH Flow Cytometry Core). .. BxPC-3 cells transiently expressing KrasG12D mutation were generated by transfecting parental BxPC-3 cells with pBabe-KrasG12D plasmid (Addgene plasmid no. 58902, a gift from C. Der) using the Lipofectamine 3000 system (Invitrogen) according to the manufacturer’s protocols. .. As a control, parental BxPC-3 were transfected, again using Lipofectamine 3000, with pBabe-Kras Wt plasmid (Addgene, plasmid no. 75282, a gift from C. Der).

    Construct:

    Article Title: A systematic genome-wide mapping of oncogenic mutation selection during CRISPR-Cas9 genome editing
    Article Snippet: .. Constructs and stable cell lines MOLM13 cells were obtained from DSMZ (Cat. ACC-554) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA) supplemented with 10% v/v heat-inactivated fetal bovine serum (Sigma-Aldrich, Saint Louis, MI), 2 mM L-Glutamine (LifeTechnologies) and 100 U/mL penicillin/streptomycin (LifeTechnologies). p53 R248Q was PCR ampli ed from a bacterial expression plasmid (kind gift of Dr. Shannon Lauberth, UCSD) and KRASG12D the pBabe-KRASG12D plasmid (Addgene plasmid 58902, from Dr. Channing Der) using the Kappa Hi- delity DNA polymerase (Kappa Biosystems). ..

    Stable Transfection:

    Article Title: A systematic genome-wide mapping of oncogenic mutation selection during CRISPR-Cas9 genome editing
    Article Snippet: .. Constructs and stable cell lines MOLM13 cells were obtained from DSMZ (Cat. ACC-554) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA) supplemented with 10% v/v heat-inactivated fetal bovine serum (Sigma-Aldrich, Saint Louis, MI), 2 mM L-Glutamine (LifeTechnologies) and 100 U/mL penicillin/streptomycin (LifeTechnologies). p53 R248Q was PCR ampli ed from a bacterial expression plasmid (kind gift of Dr. Shannon Lauberth, UCSD) and KRASG12D the pBabe-KRASG12D plasmid (Addgene plasmid 58902, from Dr. Channing Der) using the Kappa Hi- delity DNA polymerase (Kappa Biosystems). ..

    Polymerase Chain Reaction:

    Article Title: A systematic genome-wide mapping of oncogenic mutation selection during CRISPR-Cas9 genome editing
    Article Snippet: .. Constructs and stable cell lines MOLM13 cells were obtained from DSMZ (Cat. ACC-554) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA) supplemented with 10% v/v heat-inactivated fetal bovine serum (Sigma-Aldrich, Saint Louis, MI), 2 mM L-Glutamine (LifeTechnologies) and 100 U/mL penicillin/streptomycin (LifeTechnologies). p53 R248Q was PCR ampli ed from a bacterial expression plasmid (kind gift of Dr. Shannon Lauberth, UCSD) and KRASG12D the pBabe-KRASG12D plasmid (Addgene plasmid 58902, from Dr. Channing Der) using the Kappa Hi- delity DNA polymerase (Kappa Biosystems). ..

    Expressing:

    Article Title: A systematic genome-wide mapping of oncogenic mutation selection during CRISPR-Cas9 genome editing
    Article Snippet: .. Constructs and stable cell lines MOLM13 cells were obtained from DSMZ (Cat. ACC-554) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA) supplemented with 10% v/v heat-inactivated fetal bovine serum (Sigma-Aldrich, Saint Louis, MI), 2 mM L-Glutamine (LifeTechnologies) and 100 U/mL penicillin/streptomycin (LifeTechnologies). p53 R248Q was PCR ampli ed from a bacterial expression plasmid (kind gift of Dr. Shannon Lauberth, UCSD) and KRASG12D the pBabe-KRASG12D plasmid (Addgene plasmid 58902, from Dr. Channing Der) using the Kappa Hi- delity DNA polymerase (Kappa Biosystems). ..

    Article Title: Therapeutically reprogrammed nutrient signalling enhances nanoparticulate albumin bound drug uptake and efficacy in KRAS-mutant cancer.
    Article Snippet: KP or iKras cells stably expressing ERK-KTR, referred to as KP ERK-KTR or iKras ERK-KTR, respectively, were selected with growth media containing 10 μg ml−1 puromycin (Invitrogen) for 7 d and subsequently sorted for cells with high expression of fluorescent proteins via fluorescence activated cell sorting (FACS, MGH Flow Cytometry Core). .. BxPC-3 cells transiently expressing KrasG12D mutation were generated by transfecting parental BxPC-3 cells with pBabe-KrasG12D plasmid (Addgene plasmid no. 58902, a gift from C. Der) using the Lipofectamine 3000 system (Invitrogen) according to the manufacturer’s protocols. .. As a control, parental BxPC-3 were transfected, again using Lipofectamine 3000, with pBabe-Kras Wt plasmid (Addgene, plasmid no. 75282, a gift from C. Der).

    Mutagenesis:

    Article Title: Therapeutically reprogrammed nutrient signalling enhances nanoparticulate albumin bound drug uptake and efficacy in KRAS-mutant cancer.
    Article Snippet: KP or iKras cells stably expressing ERK-KTR, referred to as KP ERK-KTR or iKras ERK-KTR, respectively, were selected with growth media containing 10 μg ml−1 puromycin (Invitrogen) for 7 d and subsequently sorted for cells with high expression of fluorescent proteins via fluorescence activated cell sorting (FACS, MGH Flow Cytometry Core). .. BxPC-3 cells transiently expressing KrasG12D mutation were generated by transfecting parental BxPC-3 cells with pBabe-KrasG12D plasmid (Addgene plasmid no. 58902, a gift from C. Der) using the Lipofectamine 3000 system (Invitrogen) according to the manufacturer’s protocols. .. As a control, parental BxPC-3 were transfected, again using Lipofectamine 3000, with pBabe-Kras Wt plasmid (Addgene, plasmid no. 75282, a gift from C. Der).

    Generated:

    Article Title: Therapeutically reprogrammed nutrient signalling enhances nanoparticulate albumin bound drug uptake and efficacy in KRAS-mutant cancer.
    Article Snippet: KP or iKras cells stably expressing ERK-KTR, referred to as KP ERK-KTR or iKras ERK-KTR, respectively, were selected with growth media containing 10 μg ml−1 puromycin (Invitrogen) for 7 d and subsequently sorted for cells with high expression of fluorescent proteins via fluorescence activated cell sorting (FACS, MGH Flow Cytometry Core). .. BxPC-3 cells transiently expressing KrasG12D mutation were generated by transfecting parental BxPC-3 cells with pBabe-KrasG12D plasmid (Addgene plasmid no. 58902, a gift from C. Der) using the Lipofectamine 3000 system (Invitrogen) according to the manufacturer’s protocols. .. As a control, parental BxPC-3 were transfected, again using Lipofectamine 3000, with pBabe-Kras Wt plasmid (Addgene, plasmid no. 75282, a gift from C. Der).



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